home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Software Vault: The Sapphire Collection
/
Software Vault (Sapphire Collection) (Digital Impact).ISO
/
cdr16
/
med9410e.zip
/
M94B0802.TXT
< prev
next >
Wrap
Text File
|
1994-11-11
|
2KB
|
31 lines
Document 0802
DOCN M94B0802
TI Ribozyme targeting of HIV-1 LTR.
DT 9412
AU Ventura M; Wang P; Franck N; Saragosti S; ICGM, INSERM U 363, Universite
Paris V, Hospital Cochin, Paris,; France.
SO Biochem Biophys Res Commun. 1994 Sep 15;203(2):889-98. Unique Identifier
: AIDSLINE MED/94380073
AB The 5'-TAR region of HIV-1 mRNA is highly conserved amongst different
HIV-1 isolates. We thus investigated the potential for in vivo targeting
of the TAR RNA element by a hammerhead ribozyme. The use of the CAT
reporter gene linked to the HIV1-LTR, in transient assays, reveals that
a hammerhead ribozyme directed towards the first GUC of HIV-1 mRNA can
efficiently inhibit CAT protein expression. We show that this inhibition
is sequence-specific and probably due to a cleavage activity rather than
an antisense effect. We show also that a hammerhead ribozyme that is
inactive in vitro is capable of inhibiting CAT protein expression in a
cellular environment. These results suggest that the targeting of the
HIV-1 LTR by a hammerhead ribozyme constitutes a viable approach for
anti-HIV therapy.
DE Base Sequence Cell Line Chloramphenicol Acetyltransferase/GENETICS
Gene Expression Gene Products, tat/GENETICS/PHARMACOLOGY Genes,
Reporter HIV Long Terminal Repeat/*GENETICS HIV-1/*GENETICS Molecular
Sequence Data Plasmids RNA, Catalytic/ANTAGONISTS &
INHIB/CHEMISTRY/*METABOLISM RNA, Messenger/METABOLISM Transfection
JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).